Evaluation of Polymerase Chain Reaction Conditions and Primer Specificity for Plasmodium vivax 18S rRNA gene Detection Across Different Parasite Life Stages

Ganjar Noviar; Alda Rahmah; Indra Lesmana Rahayu; Fusvita Merdekawati; Sulaeman; Asep Iin Nur Indra

Evaluation of Polymerase Chain Reaction Conditions and Primer Specificity for Plasmodium vivax 18S rRNA gene Detection Across Different Parasite Life Stages

Authors

Ganjar Noviar Medical Laboratory Technologist, Politeknik Kesehatan Bandung https://orcid.org/0009-0001-8748-4153
Alda Rahmah Medical Laboratory Technologist, Politeknik Kesehatan Bandung https://orcid.org/0009-0006-8611-4149
Indra Lesmana Rahayu Medical Laboratory Technologist, Politeknik Kesehatan Bandung https://orcid.org/0000-0001-5399-1096
Fusvita Merdekawati Medical Laboratory Technologist, Politeknik Kesehatan Bandung https://orcid.org/0009-0000-9293-9610
Sulaeman Medical Laboratory Technologist, Politeknik Kesehatan Bandung https://orcid.org/0009-0009-3298-0297
Asep Iin Nur Indra Medical Laboratory Technologist, Politeknik Kesehatan Bandung https://orcid.org/0009-0005-9235-7932

Keywords:

Malaria, Plasmodium vivax, 18S rRNA gene, Denaturation,, PCR

Abstract

Introduction: Malaria caused by Plasmodium vivax remains widespread, with dormant liver stages that can trigger relapses. This study aimed to optimize denaturation and extension temperatures for qPCR detection of the P. vivax 18S rRNA gene, following previous optimization of primer concentration and annealing temperature.

Downloads

Download data is not yet available.

Downloads

PDF (Español (España))

Published

2026-01-29

How to Cite

Noviar, G., Rahmah, A., Rahayu, I. L., Merdekawati, F., Sulaeman, & Nur Indra, A. I. (2026). Evaluation of Polymerase Chain Reaction Conditions and Primer Specificity for Plasmodium vivax 18S rRNA gene Detection Across Different Parasite Life Stages. Gaceta Médica De Caracas, 134(Supl. 1), S125-S134. Retrieved from https://saber.ucv.ve/ojs/index.php/rev_gmc/article/view/32009

Download Citation

Issue

Vol. 134 No. Supl. 1 (2026)

Section

ARTÍCULOS ORIGINALES

License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Evaluation of Polymerase Chain Reaction Conditions and Primer Specificity for Plasmodium vivax 18S rRNA gene Detection Across Different Parasite Life Stages

Authors

Keywords:

Abstract

Downloads

Downloads

Published

How to Cite

Issue

Section

License

Most read articles by the same author(s)

Similar Articles

Information

Language

Make a Submission

Current Issue