Evaluation of Polymerase Chain Reaction Conditions and Primer Specificity for Plasmodium vivax 18S rRNA gene Detection Across Different Parasite Life Stages

Authors

Keywords:

Malaria, Plasmodium vivax, 18S rRNA gene, Denaturation,, PCR

Abstract

Introduction: Malaria caused by Plasmodium vivax remains widespread, with dormant liver stages that can trigger relapses. This study aimed to optimize denaturation and extension temperatures for qPCR detection of the P. vivax 18S rRNA gene, following previous optimization of primer concentration and annealing temperature.

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Published

2026-01-29

How to Cite

Noviar, G., Rahmah, A., Rahayu, I. L., Merdekawati, F., Sulaeman, & Nur Indra, A. I. (2026). Evaluation of Polymerase Chain Reaction Conditions and Primer Specificity for Plasmodium vivax 18S rRNA gene Detection Across Different Parasite Life Stages. Gaceta Médica De Caracas, 134(Supl. 1), S125-S134. Retrieved from https://saber.ucv.ve/ojs/index.php/rev_gmc/article/view/32009

Issue

Vol. 134 No. Supl. 1 (2026)

Section

ARTÍCULOS ORIGINALES

License

Copyright (c) 2026 Gaceta Médica de Caracas

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

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