Temperature and Time Optimization of pGEM-T Plasmid Transformation in Escherichia coli JM109
Keywords:
Recombinant, technology, transformation, Escherichia coli JM109, imageJAbstract
Introduction: The widely used method for transformation was the heat shock method, which was influenced by the time and temperature of heat shock treatment. However, there was a lack of research on the optimal temperature for pGEM-T plasmid
transformation in Escherichia coli JM109. This study uses the heat shock method to determine the optimum temperature and time for pGEM-T transformation in Escherichia coli JM109.
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Published
2023-08-23
How to Cite
Merdekawati, F. ., Manurung, J. E. ., & Nurhayati, B. . (2023). Temperature and Time Optimization of pGEM-T Plasmid Transformation in Escherichia coli JM109. Gaceta Médica De Caracas, 131(4S). Retrieved from https://saber.ucv.ve/ojs/index.php/rev_gmc/article/view/27109
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Section
ARTÍCULOS ORIGINALES
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Copyright (c) 2023 Gaceta Médica de Caracas
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
