Saber UCV
Repositorio Institucional
Repositorio Institucional
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https://saber.ucv.ve/handle/10872/15658
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| Title: | Disruption of Ca2 + Homeostasis in Trypanosoma cruzi by Crystal Violet |
| Authors: | DOCAMPO, ROBERTO GADELHA, FERNANDA R. MORENO, SILVIA N. J. BENAÍM, GUSTAVO HOFFMANN, MARIA E. VERCESI, ANIBAL E. |
| Keywords: | Mitochondria plasma membrane Ca2+-ATPase fura-2 crystal violet mitochondrial membrane Trypanosoma cruzi epimastigotes |
| Issue Date: | 1993 |
| Publisher: | The Journal of eukaryotic microbiology |
| Series/Report no.: | Vol. 40;No. 3 pp 311-316 |
| Abstract: | We have demonstrated previously that crystal violet induces a rapid, dose-related collapse of the inner mitochondrial membrane potential of Trypanosoma cruzi epimastigotes. In this work, we show that crystal violet-induced dissipation of the membrane potential was accompanied by an efflux of Ca2+ from the mitochondria. In addition, crystal violet inhibited the ATP-dependent, oligomycin-, and antimycin A-insensitiveCa2+ uptake by digitonin-permeabilized epimastigotes. Crystal violet also induced Ca2+ release from the mitochondria and endoplasmic reticulum of digitonin-permeabilized trypomastigotes. Furthermore, crystal violet inhibited Ca2+ uptake and the (Ca2+ -Mg2+)-ATPase of a highly enriched plasma membrane fraction of epimastigotes, thus indicating an inhibition of other calcium transport mechanisms of the cells. Disruption of Ca2+ homeostasis by crystal violet may be a key process leading to trypanosome cell injury by this drug. |
| URI: | http://hdl.handle.net/10872/15658 |
| ISSN: | 1550-7408 (Electronic) 1066-5234 (Linking) 1066-5234 (Print) |
| Appears in Collections: | Artículos Publicados |
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