Efecto del manejo farmacológico del sistema nervioso autónomo en la pancreatitis aguda inducida por el veneno de escorpión Tityus zulianus en un modelo ex vivo

Katiuska María Saravia Cruces; Itala América Lippo de Bécemberg; Ernesto José Trejo; Marcelo Alfonzo Rosas; Zury Domínguez

Authors

Katiuska María Saravia Cruces Instituto de Medicina Experimental. Universidad Central de Venezuela. Caracas, Venezuela
Itala América Lippo de Bécemberg Facultad de Medicina. Universidad Central de Venezuela. Caracas, Venezuela
Ernesto José Trejo Cátedra de Patología General y Fisiopatología. Escuela de Medicina Luis Razetti-Facultad de Medicina. Universidad Central de Venezuela. Caracas, Venezuela
Marcelo Alfonzo Rosas Universidad Central de Venezuela. Caracas, Venezuela
Zury Domínguez Cátedra de Patología General y Fisiopatología. Escuela de Medicina Luis Razetti. Instituto de Medicina Experimental. Universidad Central de Venezuela. Caracas, Venezuela

Keywords:

Envenenamiento escorpiónico, Tityus zulianus, pancreatitis aguda, Sistema Nervioso Simpático, agonistas adrenérgicos.

Abstract

Introduction: Pancreatitis is one of the main consequences of scorpionic poisoning produced by the genus Tityus. The pharmacological management through the use of agonists and α adrenergic antagonists in experimental models in vivo and in vitro, allow us to establish an approximation of the role of the Sympathetic Nervous System (SNS) in the development of pancreatitis. Objective: to determine the role of SNS in the development of acute pancreatitis induced by the scorpion venom Tityus zulianus (TzV), through the use of sympatholytics such as clonidine and prazosin. Methods: Plasma Protein Extravasation (PPE) in the pancreas was evaluated by the method of Evans Blue (EA), modified by Saria and Lundberg (1983) at 620 nm; n = 3 NIH mice in each experimental group. Comparisons were made by one-way ANOVA and post-HOC tests by Tukey-Kramer. Results: Both drugs (1mg / Kg) significantly decreased p <0.01 (**) the EPP in the pancreas induced by TzV, compared to animals inoculated only with TzV. There were no significant differences between the animals in the control group and the groups treated with drugs plus TzV. Conclusions: The pancreatotoxic effect of TzV in mice could have an autonomic component since sympatholytic drugs by decreasing noradrenergic activity reduce the magnitude of edema. This suggests that both drugs can be used as a therapeutic strategy in these cases.

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