Saber UCV
Repositorio Institucional
Repositorio Institucional
Please use this identifier to cite or link to this item:
https://saber.ucv.ve/handle/10872/6839
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| Title: | Cloning and sequencing of c-DNA encoding N-methyl-D-aspartate receptor |
| Authors: | Ruiz E., Ana Z. |
| Keywords: | cloning NMDA receptor Ovis aries ram sequencing |
| Issue Date: | 1-Apr-2014 |
| Abstract: | The objective of the present study was to report the molecular cloning and determination of the sequences N-methyl-D-aspartate subunit-1 receptor (NMDAR-1) in the hypothalamus of sheep. The re-amplified DNA template for NMDA -1 from the hypothalamus of male sheep was cloned using pBluescript-Sk-plasmids (pBSK, 2958 bp). Purified plasmids containing the NMDA receptor c-DNA were sequenced using the dye-terminator chemistry at the University of Nebraska- Lincoln, Nebraska, USA. Results were entered into the National Center for Biotechnology Web site as accession number AY434689. The sequence of Ovis aries (sheep) NMDAR-1 mRNA from hypothalamus of male sheep has 94-97% homology with Homo sapiens, 94-100% homology with Sus scrofa, and 88-90% homology with Rattus norvegicus NMDAR-1 mRNA sequence, and 94-97% homology with Homo sapiens glutamate receptor and transcript variant NR1-2 and NR1-1 mRNA sequence. These results show high evolutionary conservation of NMDA receptor subunit-1 across species. |
| URI: | http://hdl.handle.net/10872/6839 |
| Appears in Collections: | Artículos Publicados |
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