Saber UCV
Repositorio Institucional
Repositorio Institucional
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https://saber.ucv.ve/handle/10872/5144
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| Title: | The effects of Lonomin V, a toxin from the caterpillar (Lonomia achelous), on hemostasis parameters as measured by platelet function |
| Authors: | Guerrero, Belsy Arocha-Piñango, Carmen L. Salazar, Ana M. Gil, Amparo Sánchez, Elda E. Rodríguez-Acosta, Alexis Lucena, Sara |
| Keywords: | Lonomia achelous caterpillar Lonomin V Platelets Primary hemostasis |
| Issue Date: | 29-Nov-2013 |
| Series/Report no.: | Toxicon;58 (2011) 293–303 |
| Abstract: | Platelets play a central role in hemostasis during vascular injury. Patients affected with the hemorrhagic syndrome caused by contact with Lonomia achelous caterpillars (Lac) Lepi- doptera distributed in various South American countries, show digestive, pulmonary and intraperitoneal bleeding in combination with hematomas and echymosis. In the present study, we have evaluated the effects of Lonomin V (serine protease isolated from Lac hemolymph) on some functional properties of platelets, evaluating its importance in primary hemostasis. Platelet adhesion to fibrinogen was reduced by 19, 20, 36, and 37% after pre-treated with 0.2, 2, 20 and 40 nM of Lonomin V, respectively. Pre-incubation of the platelets with 408 nM of Lonomin V, for 4 min at 37 C, resulted in complete inhibition of the collagen-induced platelet aggregation, in contrast to 56% inhibition of the ADP - induced platelet aggregation. Lonomin V also inhibited anti-aIIbb3 integrin binding to platelets by 56, 57, 52 and 54% at concentrations of 0.2, 2, 20 and 40 nM respectively. Additionally, Lonomin V inhibited anti-P-selectin binding to platelets by 28, 37, 33 and 33% at the same concentrations. The platelets tested with Lonomin V did not modify their viability. In summary, Lonomin V inhibited platelet aggregation, probably caused by the degradation of collagen. The anti-platelet activity of Lonomin V has been shown to be unique and a potentially useful tool for investigating cell-matrix and cell–cell interactions and for the development of antithrombotic agents in terms of their anti-adhesive activities. |
| URI: | http://hdl.handle.net/10872/5144 |
| Appears in Collections: | Artículos Publicados |
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