Saber UCV
Repositorio Institucional
Repositorio Institucional
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https://saber.ucv.ve/handle/10872/5127
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| Title: | Inhibition of Lung Tumor Colonization and Cell Migration with the Disintegrin Crotatroxin 2 Isolated from the Venom of Crotalus atrox |
| Authors: | Galán, Jacob A Sánchez, Elda E Rodríguez-Acosta, Alexis Soto, Julio G Bashir, Sajid McLane, Mary Ann Paquette-Straub, Carrie Pérez, John C |
| Keywords: | lung tumor colonization cell migration, Crotalus atrox crotatroxin disintegrins venom |
| Issue Date: | 28-Nov-2013 |
| Series/Report no.: | Toxicon;51(7):1186-1196. (2008). |
| Abstract: | Disintegrins are low molecular weight proteins (4-15 kDa) with an RGD binding region at their binding loop. Disintegrin and disintegrin-like proteins are found in the venom of four families of snakes: Atractaspididae, Elapidae, Viperidae and Colubridae. This report describes the biological activity of a disintegrin, crotatroxin 2, isolated by a three-step chromatography procedure from the venom of the Western diamondback rattlesnake (Crotalus atrox). The intact molecular mass for crotatroxin 2 was 7.384 kDa and 71 amino acids. Crotatroxin 2 inhibited human whole blood platelet aggregation with an IC50 of 17.5 nM, inhibited cell (66.3p) migration by 63%, and inhibited experimental lung tumor colonization in BALB/c mice at 1000 μg/kg. Our data suggest that while crotatroxin 2 inhibits platelet aggregation, cancer cell migration, and lung tumor colonization it is done via different integrins |
| URI: | http://hdl.handle.net/10872/5127 |
| Appears in Collections: | Artículos Publicados |
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