Embrión de Pollo: Un modelo con cardiovideograma para el estudio de la toxicidad experimental inducida por Thevetia peruviana.

Abstract

Studies on the cardiotoxicity induced by chemical agents have been strengthened through the use of cardiogenic signals registration methods. In this study we used a method, defined in our laboratory as Cardiovideogram (CVG), reinforced with image analysis, to illustrate “in vivo” chick embryo heart the cardiotoxicity induced by aqueous extract of Thevetia Peruvians seeds, an ornamental plant with severe toxic action. The results showed that the aqueous extract of Thevetia Peruvians seeds induces Average Heart Rate (AHR) reduction in 70.49% measured in beats per minute (P/ min) through variations of brightness measurement in intervals between the peaks of this variation. These changes were associated with a higher Ventricular (V) and Truncus Arteriosus (TA) systolic level and a lower Atrial (A) systole level, compared to the control condition. It was shown that the cardiotoxicity induced by the aqueous extract of Thevetia Peruvians seed can be clearly analyzed in chick embryo cardiac model, strengthened with Cardiovodeogram (CVG) which is an alternative recording method for study of many other toxic agents with cardiotoxic potentially