Enzymatic Activity of Lactoperoxidase in the Colostrum of Cows in different lactations

Abstract

In order to evaluate the activity of the lactoperoxidase enzyme (LPO), in colostrum and milk at the first month of lactation, the characterization of colostrum in cows was carried out from the first day postpartum and during the first month of lactation, in first, second, third and more lactations for six (06) months, the parameters were analyzed: acidity, pH, temperature, qualitative detection of the lactoperoxidase enzyme, quantifying the volume of the LPO activity. Descriptive statistics were applied using the STATISTIX 4.0 package (1985-1992) and one way nonparametric statistics Kruskal – Wallis test for the levels First, second, third and more lactations and for the characterized parameters, using the SAS-STAT statistical system, to know the independence of the variables under study. It was found that the acidity is high and varies in a decreasing manner as follows: 37.68; 27.13 and 26.13 ml of NaOH O.1N/100ml. respectively, the pH was observed below that of milk, ranging between 5.0 and 6.2 (equiv/L). The enzymatic activity registered values of up to 182.89 U/lt., considered low compared to milk in the first month of lactation; from one to more than three lactations, according to the values found: 383.9, 394.39 and 753.54 U/ml respectively. It is concluded that the enzymatic activity is low in colostrum and then increases, until reaching the values of normal milk in the first month of lactation. The independence of each parameter (variable) in colostrum was also verified.