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> Isolation of Fungi and Gram Negative Bacteria from Toothbrushes and Bathroom Bioaerosols
Please use this identifier to cite or link to this item: https://saber.ucv.ve/handle/10872/18552

Title: Isolation of Fungi and Gram Negative Bacteria from Toothbrushes and Bathroom Bioaerosols
Authors: Merino-Alado, Rommie
Garcés, Alessandra
Chianale, Elisa
Corcuera, Carlos
El Fakih, Wanda
Galviz, Dolly
Ortiz, Lisbeth
Sucre Campins, Alfredo
Moronta, Gustavo
Briceño, Elsi
Landaeta, María Eugenia
Mata-Essayag, Sofia
Keywords: toothbrushes
enterobacteriaceae
Issue Date: 26-May-2018
Series/Report no.: Pesquisa Brasileira em Odontopediatria e Clinica Integrada;2018, 18(1):e3994
Abstract: Objective: To identify, using phenotypic methods, FGNB, NFGNB and Candida sp. in toothbrushes, and environmental samples of bathroom air in a group of students from the Dentistry School of the Universidad Central de Venezuela. Material and Methods: Thirty-four toothbrushes were supplied to the cohort during a 60-day period; environmental samples were collected in the rooms where toothbrushes were kept during this period. All samples were processed by traditional methods of microbiological counting isolation and phenotypic identification using selective and differential agar based on the international guidelines of the United States Pharmacopeia (USP) 38. Results: 82.36% of the toothbrush samples were positive to bacteria and fungi and 91.17% of the environmental samples were positive to enterobacteria. Conclusion: It is necessary to establish antiseptic protocols for the management, storage and disinfection of toothbrushes. The high rate of contamination may represent an opportunity for enterobacteria colonization of oral biofilms, reservoir to infection foci and metastatic infections in certain populations.
URI: http://hdl.handle.net/10872/18552
ISSN: 1519-0501
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