Saber UCV
Repositorio Institucional
Repositorio Institucional
Please use this identifier to cite or link to this item:
https://saber.ucv.ve/handle/10872/17717
|
| Title: | Vitamin D and L-Isoleucine Promote Antimicrobial Peptide hBD-2 Production in Peripheral Blood Mononuclear Cells from Elderly Individuals |
| Authors: | Araujo, Zaida Castañeda-Delgado, Julio E. Gonzalez-Curiel, Irma Serrano, Carmen J. Rivas Santiago, Cesar Enciso-Moreno, Jose A. Rivas-Santiago, Bruno |
| Keywords: | Defensins elderly aging vitamin D L-isoleucine innate immunity |
| Issue Date: | 10-Mar-2016 |
| Citation: | Araujo. Z Et All Vitamin D and L-Isoleucine Promote Antimicrobial Peptide hBD-2 Production in Peripheral Blood Mononuclear Cells from Elderly Individuals Int J Vitam Nutr Res (2017), 1–8 |
| Abstract: | Elderly individuals are susceptible to develop infectious diseases; promoting innate immunity to prevent infections is a key issue. Human β-defensin-2 (hBD-2) is an antimicrobial peptide with antimicrobial and immunomodulatory properties. L-isoleucine and vitamin D are important molecules that induce hBD-2. The Aim of this study was to determine the use L-isoleucine and Vitamin D to induce hBD-2 in cells from healthy elderly individuals and elderly individuals with recurrent infections. We explored three groups: young adults (n = 20) used as control group, elderly adults (n = 18) and elderly with recurrent infections (n = 11). PBMCs (peripheral blood mononuclear cells) were isolated from the different groups and then were treated with L-isoleucine or vitamin D3. hBD-2 concentration was assessed with a sandwich enzyme Immunosorbent assay by triplicate. Using the vehicle as a mock control. Our results showed that a percentage of the individuals responded to the treatments producing hBD-2 (p < 0.05). These results showed that both molecules induced hBD-2 in elderly individuals and can be potentially used as prophylactic therapy to decrease infection diseases rates in this vulnerable group. |
| URI: | http://hdl.handle.net/10872/17717 |
| Appears in Collections: | Artículos Publicados |
Files in This Item:
|
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.