Saber UCV
Repositorio Institucional
Repositorio Institucional
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https://saber.ucv.ve/handle/10872/16416
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| Title: | In vitro levels of cytokines in response to purified protein derivative (PPD) antigen in a population with high prevalence of pulmonary tuberculosis |
| Authors: | Giampietro, Francesca De Waard, Jacobus H. Rivas- Santiago, Bruno Encimo- Moreno, Jose Antonio Salgado, Antonio Araujo, Zaida |
| Keywords: | TB IL PBMCs PPD |
| Issue Date: | Jul-2010 |
| Publisher: | Human Immunology |
| Series/Report no.: | vol. 71; |
| Abstract: | The control of mycobacterial infection by the host depends on cell-mediated immunity (CMI), involving activated macrophages, T cells, and type 1 cytokines (Th1). Here we evaluated the capacity of antigeninduced proliferation by peripheral blood mononuclear cells (PBMCs) and the production of proinflammatory tumor necrosis factor-alpha (TNF- ) and interleukin 12 (IL-12p40). The Th1 cytokine interferon-gamma (IFN– ) and Th2 cytokines interleukin 4 (IL-4) and interleukin 5 (IL-5) in 62 pulmonary tuberculosis (TB) patients (40 Warao indigenous patients [WP] and 22 Creole nonindigenous patients [CP]) and 24 healthy controls (12 Warao indigenous controls [WC] and 12 Creole nonindigenous controls [CC]) at 24 and 48 hours in response to purified protein derivative (PPD) from Mycobacterium tuberculosis. The overall results revealed that testing of CP and CC’ PBMCs for TNF- , IFN- , and IL-12p40 production was higher compared with WP and WC’ PBMCs after stimulating for 24 and 48 hours (p 0.0001), within the WP group, the lower productions of IL-12p40 and IFN- significantly correlated (r2 0.91, p 0.01). Although in general there was interindividual variability in the observed responses of Th2 cytokines, especially with IL-4, there was a trend to produce higher PPD-induced IL-5 by WP’ PBMCs compared with WC’ PBMCs and CP’ PBMCs at 24 and 48hours, respectively. High IL-5 production correlated inversely with low IFN- production (r2 0.97, p 0.002). In conclusion, our results suggest that PPD-induced responses observed in patients from both populations can be divided into two groups: one group that activates Creole’ PBMCs to preferentially secrete TNF- , IL-12p40 and IFN- and another group that activates preferential secretion of IL-5 in Warao’ PBMCs.2010 American Society for Histocompatibility and Immunogenetics. Published by Elsevier Inc. All rights reserved. |
| URI: | http://hdl.handle.net/10872/16416 |
| ISSN: | 1099–1104 |
| Appears in Collections: | Artículos Publicados |
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