Saber UCV
Repositorio Institucional
Repositorio Institucional
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https://saber.ucv.ve/handle/10872/16365
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| Title: | Study of the Antibody Response against Mycobacterium tuberculosis Antigens in Warao Amerindian Children in Venezuela |
| Authors: | Araujo, Zaida De Waard, Jacobus H. Fernandez De Larrea, Carlos Lopez, Diana Fandiño, C. Maldonado, A. Hernadez, E. Ocaña, Yelka Ortega, R. Singh, M. Ottenhoff, Tom H. Arend, SM Convit, Jacinto |
| Keywords: | Tuberculosis (TB) Warao Amerindians Tuberculin skin test (TST) IgA lgG IgE Secretory 19A (sIgA) |
| Issue Date: | Aug-2004 |
| Publisher: | Mem Inst Oswaldo Cruz, Rio de janeiro, |
| Series/Report no.: | Vol.99;5 |
| Abstract: | This study was aimed at investigating alternate methods for serodiagnosis of tuberculosis (TB), which are needed because bacteriologic diagnosis of childhood TB is difficult. A selection of 80 serum and saliva samples were tésted from Warao indigenous children under 15 years of age; 34 high TB suspects (28 positive and 6 negative for the tuberculin skin test, TST) and 46 healthy Contact children (32 positive and 14 hégative for the TST). Several enzyme-rinked immunosorbent assay (ELISA) serological tests were developed to test for Mycobacterium tuberculosis- specific antibodies, including serum IgA, IgG; IgE, and secretory IgA (sIgA) in saliva against 3 specific antigens (PPD, HSP60, 38 kDa). Of these, 2 antigens, P.PD and 38 kDa, showed significantly higher reactivity. The sensitivity and specificity of these tests for diagnosis remained limited, between 26.5% and 38.2%, and 77.4% and 97%, respectively. Of all the samples studied and combinations realized between all isotypes and antigens combined with 3 isotypes (anti-PPD IgG; IgE, and anti-38kDa sIgA) man{lged to detect the largest number of patients, showing an improved sehsitivity level of 64.7%, although specificity levels dropped to 81.8%. These results were compared with the Omega diagnostics commercial kit results. The commercial kits showed significantly lower reactivity (sensitivity of20% and 13.33% to Myco G dnd Complex Plus, respectively) anda specificity óf 100%. This study shows that in indigenous populations of Ve1'Íezuelaw, here invasive procedures cannot be used to select samples but evaluation with a chest X-ray for radiological studies is ávailab!e, the combination of 3 specific isotypes may be a useful tool to increase diagnostic accurac)1 with pulmonary TB in this population, when used together with clinical and epidemiologic(ll criteria. |
| URI: | http://hdl.handle.net/10872/16365 |
| ISSN: | 0034-8023 |
| Appears in Collections: | Artículos Publicados |
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