Saber UCV
Repositorio Institucional
Repositorio Institucional
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https://saber.ucv.ve/handle/10872/16118
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| Title: | Plant regeneration of Anthurium andreanum cv Rubrun |
| Authors: | Vargas, Teresa E. Mejías, Alexander Oropeza, Maira García, Eva |
| Keywords: | Callus micropropagation organogenesis. old in vitro plants |
| Issue Date: | 2004 |
| Citation: | Vargas, T. E., Mejías, A., Oropeza, M., & García, E. (2004). Plant regeneration of Anthurium andreanum cv Rubrun. Electronic Journal of Biotechnology, 7(3). Chile: Pontificia Universidad Católica de Valparaíso |
| Abstract: | To establish an efficient regeneration system for Anthurium andreanum cv Rubrun, seeds from plant spadixes were germinated on a medium supplemented with 2.2 m M BA. After 2 weeks, 74% of the sedes germinated and four weeks later, micro-cuttings from these plantlets were subcultured on a médium containing 4.4 m M BA and 0.05 m M NAA. On average, 3.6 shoots per explant were obtained. Four weeks old in vitro plants from germinated seeds and the plantlets obtained from micro-cuttings, showed callus proliferation at the stem base. These tissues were subcultured on a medium supplemented with 8.9 m M BA and 2.7 m M NAA. After 6 weeks of culture, about 43.8 plantlets per square cm of callus were obtained. Anatomical studies showed the organogenic nature of these calli. Anthurium andreanum plants regenerated by organogenesis were transferred to pots and a rate of 80% of plant acclimatization was obtained. |
| Description: | Teresa E. Vargas: Laboratorio de Biotecnología Vegetal Instituto de Biología Experimental, Facultad de Ciencias Universidad Central de Venezuela Caracas, Apartado 47114 Los Chaguaramos, Venezuela Tel: 58 212 7510111 Fax: 58 2127535897 E-mail: jojoagon@hotmail.com. Alexander Mejías: Laboratorio de Biotecnología Vegetal Instituto de Biología Experimental, Facultad de Ciencias Universidad Central de Venezuela Caracas, Apartado 47114 Los Chaguaramos, Venezuela Tel: 58 212 7510111 Fax: 58 2127535897 E-mail: alexandermejias@hotmail.com. Maira Oropeza: Laboratorio de Biotecnología Vegetal Instituto de Biología Experimental, Facultad de Ciencias Universidad Central de Venezuela Caracas, Apartado 47114 Los Chaguaramos, Venezuela Tel: 58 212 7510111 Fax: 58 2127535897 E-mail: moropeza@strix.ciens.ucv.ve. Eva de García: Laboratorio de Biotecnología Vegetal Instituto de Biología Experimental, Facultad de Ciencias Universidad Central de Venezuela Caracas, Apartado 47114 Los Chaguaramos, Venezuela Tel: 58 212 7510111 Fax: 58 2127535897 E-mail: egarcia@reacciun.ve |
| URI: | http://hdl.handle.net/10872/16118 |
| ISSN: | 0717-3458 |
| Appears in Collections: | Artículos Publicados |
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