Analysis of a Musa spp. somaclonal variant resistant to yellow Sigatoka

Abstract

Somaclonal variant CIEN BTA-03 resistant to yellow Sigatoka was obtained from a susceptible banana clone (Williams clone), by increasing the production of adventitious buds using 6-Belcilaminopurine at high concentrations. This somaclone has exhibited yellow Sigatoka resistance in the field for five consecutive years of asexual reproduction. We used RAPD markers to generate characteristic “fingerprints” for each probe, concluding that they are reliable tools for evaluating the genetic variability of Musa regenerants obtained by in vitro culture.