Saber UCV
Repositorio Institucional
Repositorio Institucional
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https://saber.ucv.ve/handle/10872/15708
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| Title: | The role of a H+-ATPase in the regulation of cytoplasmic pH in Trypanosoma cruzi epimastigotes |
| Authors: | VANDERHEYDEN, Nicole BENAÍM, Gustavo DOCAMPO, Roberto |
| Keywords: | Cytoplasmic pH Trypanosoma cruzi omeprazole vanadate bafilomycin A1 fluorescent probe bisoxonol |
| Issue Date: | 1996 |
| Publisher: | The Biochemical journal |
| Series/Report no.: | Vol. 318;pp 103-109 |
| Abstract: | Cytoplasmic pH (pHi) regulation was studied in Trypanosoma cruzi epimastigotes using fluorescent probes. Steady-state pH1 was maintained even in the absence of extracellular Na+ or K+, but was significantly decreased in the absence of C1−. Acidloaded epimastigotes regained normal pH1 by a process that was ATP-dependent and sensitive to N-ethylmaleimide, dicyclohexylcarbodi- imide and diethylstilboestrol, suggesting involvement of a H+-pumping ATPase. Recovery from an acid load was independent of extracellular Na+ or K+ and insensitive to omeprazole, vanadate and low concentrations of bafilomycin A1. Using the fluorescent probe bisoxonol to measure the membrane potential of intact cells, acid loading of epimastigotes was shown to result in a dicyclohexylcarbodi-imide-sensitive hyperpolarization, which suggests electrogenic pumping of protons across the plasma membrane. Addition of glucose, but not of 6- deoxyglucose, produced a transient cellular acidification of possible metabolic origin, and increased the rate of recovery from an acid load. Taken together, these results are consistent with an important role of a H+-ATPase in the regulation of pH1 homoeostasis in T. cruzi. |
| Description: | Department of Veterinary Pathobiology, University of Illinois at Urbana-Champaign, 2001 S. Lincoln Ave., Urbana, IL 61801, U.S.A. |
| URI: | http://hdl.handle.net/10872/15708 |
| ISSN: | 1470-8728 (Electronic) 0264-6021 (Linking) 0264-6021 (Print) |
| Appears in Collections: | Artículos Publicados |
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