Saber UCV
Repositorio Institucional
Repositorio Institucional
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https://saber.ucv.ve/handle/10872/15699
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| Title: | Sodium-Calcium Exchanger Modulates the l-Glutamate Cai 2+ Signalling in Type-1 Cerebellar Astrocytes |
| Authors: | Rojas, Héctor Colina, Claudia Ramos, Magaly Benaím, Gustavo Jaffe, Erica Caputo, Carlo Di Polo, Reinaldo |
| Keywords: | Na+/Ca2+ exchange CICR Glutamate Glutamate transporter plasma membrane Ca2+ clearance of Cai 2+ |
| Issue Date: | 2012 |
| Publisher: | Advances in Experimental Medicine and Biology |
| Series/Report no.: | Vol. 961;pp:267-274 |
| Abstract: | We have previously demonstrated that rat cerebellar type-1 astrocytes express a very active Na+/Ca2+ exchanger which accounts for most of the total plasma membrane Ca2+ fluxes and for the clearance of Cai 2+ induce by physiological agonist. In this chapter, we have explored the mechanism by which the reverse Na+/Ca2+ exchange is involved in agonistinduced Ca2+ signalling in rat cerebellar astrocytes. Laser-scanning confocal microscopy experiments using immunofluorescence labelling of Na+/Ca2+ exchanger and RyRs demonstrated that they are highly co-localized. The most important finding presented in this chapter is that L-glutamate activates the reverse mode of the Na+/Ca2+ exchange by inducing a Na+ entry through the electrogenic Na+-glutamate co-transporter and not through the ionophoric L-glutamate receptors as confirmed by pharmacological experiments with specific blockers of ionophoric L-glutamate receptors, electrogenic glutamate transporters and the Na/Ca exchange. |
| Description: | H. Rojas; C. Colina; M. Ramos; E. Jaffe; C. Caputo; R. Di Polo. Laboratorio de Fisiología Celular, Centro de Biofísica, Instituto Venezolano de Investigaciones Científicas (IVIC), Caracas, Venezuela e-mail: rdipolo@gmail.com Gustavo. Benaim Laboratorio de Señalización Celular, Instituto de Estudios Avanzados (IDEA), Caracas, Venezuela. |
| URI: | http://hdl.handle.net/10872/15699 |
| ISSN: | 2214-8019 (Electronic) 0065-2598 (Linking) 0065-2598 (Print) DOI 10.1007/978-1-4614-4756-6_22, |
| Appears in Collections: | Artículos Publicados |
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