Saber UCV
Repositorio Institucional
Repositorio Institucional
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https://saber.ucv.ve/handle/10872/15647
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| Title: | Increased calcium permeability is not responsible for the rapid lethal effects of amphotericin B on Leishmania sp. |
| Authors: | Cohen, B. Eleazar Benaím, Gustavo Ruiz, Marie-Christine Michelangeli, Fabi6n |
| Keywords: | Amphotericin B Polyene antibiotic Calcium Leishmania Quin-2 Ethidium bromide |
| Issue Date: | 1990 |
| Publisher: | Elsevier Science Publishers B. V. (Biomedical Division) |
| Series/Report no.: | Vol. 259;No. 2 pp 286-288 |
| Abstract: | The mode of action of the polyene antibiotic amphotericin B (AmB), the drug of choice for the treatment of systemic fungal infections and visceral leishmaniasis, is still unclear. An increase in intracellular Ca2+ concentration ([Ca2+]i), toxic in many cases, has been postulated as a possible lethal mechanism for AmB. Cell permeabilization to ethidium bromide (EB) was used as a criterion of viability. Kinetics of the DNA-EB fluorescent complex formation was studied in ergosterol-containing Leishmania promastigotes. Intracellular Ca2+ concentration was measured using quin-2 fluorescence in parallel aliquots. It is shown in this work that AmB can act as an efficient Ca2+ ionophore. However, the rapid permeabilization effect induced by AmB on these cells was not dependent on an increase in [Ca2+]i. On the contrary, it was found that leishmanicidal effect of AmB was enhanced in the absence of external calcium. Furthermore, A23187 a Ca2+ ionophore did not provoke cell penneabilization to EB. |
| URI: | http://hdl.handle.net/10872/15647 |
| ISSN: | https://doi.org/10.1016/0014-5793(90)80028-H |
| Appears in Collections: | Artículos Publicados |
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