Saber UCV
Repositorio Institucional
Repositorio Institucional
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https://saber.ucv.ve/handle/10872/15643
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| Title: | The Effect of Ethanol on the Plasma Membrane Calcium Pump Is Isoform-specific |
| Authors: | Cervino, Vincenza Benaím, Gustavo Carafoli, Ernesto Guerini, Danilo |
| Keywords: | effect of ethanol isoforms plasma membrane Ca2+ ATPase baculovirus system ethanol PMCA4 protein |
| Issue Date: | 1998 |
| Publisher: | THE JOURNAL OF BIOLOGICAL CHEMISTRY |
| Series/Report no.: | Vol. 273;No. 45 pp. 29811–29815 |
| Abstract: | The effect of ethanol has been studied on four different isoforms of the plasma membrane Ca2+-ATPase expressed in Sf9 cells with the help of the baculovirus system. The PMCA2CI protein was maximally activated by 0.5% ethanol, a concentration 8–10 times lower than that needed to obtain the same effect on the PMCA4 protein or on the pump of erythrocyte membranes, which is a mixture of isoforms 1 and 4. Experiments performed with truncated pumps indicated that the stimulation by ethanol was lost if the C-terminal region between Lys1065 and Lys1161, encompassing the Calmodulin binding domain, was removed. These observations indicate that the stimulation is the result of a direct interaction of ethanol with the C-terminal regulatory domain of the Ca2+ pump. |
| URI: | http://hdl.handle.net/10872/15643 |
| ISSN: | 1083-351X |
| Appears in Collections: | Artículos Publicados |
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