Saber UCV
Repositorio Institucional
Repositorio Institucional
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https://saber.ucv.ve/handle/10872/14870
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| Title: | Increased peroxide production by polymorphonuclear cells of chronic hepatitis C virus-infected patients. |
| Authors: | Toro, Felix Conesa, Angela García, Alexis H. Bianco Colmenares, Nicolás E. De Sanctis, Juan B. |
| Keywords: | polymorphonuclear cells hepatitis C virus infection peroxide production flow cytometry intracellular hydrogen production of polymorphonuclear |
| Issue Date: | Aug-1998 |
| Publisher: | CLINICAL IMMUNOLOGY AND IMMUNOPATHOLOGY |
| Series/Report no.: | Vol. 88;No. 2 pp 169-175 |
| Abstract: | To evaluate the oxidative burst in hepatitis C virus (HCV) infection, intracellular hydrogen peroxide (H202) production of polymorphonuclear (PMN) cells isolated from 15 chronic HCV-infected patients and 11 controls was assessed by flow cytometry in a time kinetic. Under nonstimulated and phorbol myristate acetate (PMA)-stimulated conditions, H202 production was higher in HCV-infected patients than in controls (P <0.05) at the time points of 20, 30, and 40 min. A positive correlation between H202 production by PMA-stimulated cells and serum levels of alanine aminotransferase and aspartate aminotransferase was found in the HCV-infected patients (r = 0.877, P <0.01 and r = 0.9351, P <0.001, respectively). RT-PCR analysis of purified mononuclear (MN) and PMN cells from HCV-infected patients revealed the presence of HCV RNA in 60% of MN and 27% of PMN cell samples. These results suggest that a functional alteration of PMN cells is manifested in this chronic viral infection which may represent an additional factor in the development of liver lesions. |
| URI: | http://hdl.handle.net/10872/14870 |
| ISSN: | 0090-1229 |
| Appears in Collections: | Artículos Publicados |
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