Saber UCV
Repositorio Institucional
Repositorio Institucional
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https://saber.ucv.ve/handle/10872/14717
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| Title: | High-density Ficoll-Hypaque as an alternative method of eosinophil purification |
| Authors: | Conesa, Angela Pérez, Carlos Rivera, Henry Aldrey, Oscar Bianco Colmenares, Nicolás E. De Sanctis, Juan B. |
| Keywords: | normodense eosinophil high-density Ficoll-Hypaque eosinophil purification mononuclear polymorphonuclear cells anti-CD16 hypodense eosinophils leucocyte populations |
| Issue Date: | 1997 |
| Publisher: | Medical science research |
| Series/Report no.: | Vol. 25;No. 11 pp 751-755 |
| Abstract: | A new method for normodense eosinophil purification is proposed. It is based on the use of high-density Ficoll-Hypaque (density = 1.114 g mL-1) instead of dextran sedimentation plus standard Ficoll-Hypaque, before discontinuous Percoll gradient centrifugation. Eosinophils from 11 controls and 11 eosinophilic patients were studied. The density distribution of eosinophils was similar in both groups. However, eosinophilic patients showed a significant increase in the absolute count of hypodense eosinophils. Normodense eosinophil purity and recovery were significantly higher in controls as compared with eosinophilic patients (p < 0.05). High-density Ficoll-Hypaque allowed separation of mononuclear and polymorphonuclear cells with high purity and recovery using a single centrifugation step. It also reduces one step in eosinophil purification as compared to the standard method. Thirdly, contrary to the single anti-CD16 magnetic bead separation method, it allows the isolation of normodense and hypodense eosinophils. In summary, this method is economic, reliable and permits the purification of different leucocyte populations. |
| URI: | http://hdl.handle.net/10872/14717 |
| ISSN: | 0269-8951 |
| Appears in Collections: | Artículos Publicados |
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