Saber UCV
Repositorio Institucional
Repositorio Institucional
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https://saber.ucv.ve/handle/10872/14054
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| Title: | LED Xuorescence spectroscopy for direct determination of monoamine oxidase B inactivation |
| Authors: | Castillo, Jimmy Hung, Jeanette Rodríguez, María del C. Bastidas, Elsy Laboren, Ivanna Jaimes, Alba |
| Keywords: | MAO-B LED FLUORESCENCE BERBERINE |
| Issue Date: | 16-Jun-2005 |
| Publisher: | Analytical Biochemistry |
| Citation: | Analytical Biochemistry 343 (2005) 293–298 |
| Abstract: | In this work, we report an alternative assay for the determination of the inhibitory eVect on monoamine oxidase B (MAO-B) activity of probe compounds. Enzyme MAO-B exhibits Xuorescence emissions when it is excited at 412 nm. Using an inexpensive blue LED-like excitation source, we measured the quenching of Xuorescence intensity of MAO-B enzyme during the reaction with inhibitors. The applicability of the procedure is demonstrated by assays with L-deprenyl and berberine as inhibitors through the use of Xuorescence studies. The IC50 values of L-deprenyl and berberine were 0.04 and 90 M, respectively. The KI values were 0.020 and 47 M for L-deprenyl and berberine, respectively. These IC50 and KI values were similar to the values obtained with a standard method. These results demonstrate the feasibility of this method as an alternative to follow the inhibitory eVect on MAO-B. |
| URI: | http://hdl.handle.net/10872/14054 |
| ISSN: | 0003-2697 |
| Appears in Collections: | Artículos Publicados |
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