Efecto del extracto de semillas de Brassica nigra contra la proliferación hepatocelular inducida por fenobarbital como agente promotor de tumors
Palabras clave:
Proliferación hepatocelular, Fenobarbital, Semillas de Brassica.Resumen
La proliferación hepatocelular es una de las principales causas de carcinoma hepatocelular (HCC) y es un tipo de muerte relacionada con el cáncer ampliamente distribuido. Existen desafíos considerables en el tratamiento del carcinoma hepatocelular, incluida la ineficacia, los efectos adversos y la resistencia a los medicamentos disponibles. Se ha establecido que un alto consumo de vegetales Brassica está relacionado con un menor riesgo de muchos tipos de cáncer. En el estudio se extrajeron semillas de Brassica nigra; Se realizó un análisis fitoquímico del extracto, incluidas pruebas de selección fitoquímica y análisis de cromatografía de gases-espectrometría de masas (GC-MS). La actividad antiproliferativa del extracto hidroalcohólico de semillas de Brassica se estudió a 800 mg/kg y se comparó con el grupo de control (administrado con solución salina normal), el grupo de fenobarbital (fenobarbital 75 mg/kg) y el grupo combinado (extracto de Brassica 800 mg/kg + fenobarbital 75 mg/kg). El análisis GC-MS reveló la presencia del compuesto de isotiocianato. En comparación con el grupo de fenobarbital, la combinación de grupos pretratados con semillas de Brassica nigra durante 14 días y fenobarbital durante siete días provocó una reducción significativa en la expresión de Ki67 y Bcl2 que fue evidente en los portaobjetos inmunohistoquímicos. Además, los grupos de combinación mostraron una disminución sustancial en el nivel de expresión de genes relacionados con el ciclo celular, incluidos los genes de mantenimiento de minicromosomas 2 (Mcm2) y ciclina A2 (Ccna2), y una reducción significativa en el nivel de expresión de Citocromo P450, incluidos CYP2B10 y CYP2C29 en comparación con el grupo fenobarbital. El extracto de semillas de Brassica nigra mostró efectos antiproliferativos y apoptóticos en el tejido hepático, lo que sugiere que tiene un impacto beneficioso para minimizar el riesgo de cáncer de hígado.
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