Definitions of antibodies to the antitumor enzyme L-lysine-α-oxidase, the study of its immunogenic properties and allergenic activity

Abstract

The determination of antibodies to the antitumor enzyme L-lysine-α-oxidase Trichoderma harzianum Rifai F-180 was studied using enzyme-linked immunosorbent assay. It was shown that when testing the enzyme in mice and guinea pigs at a dose of 35 U / kg, the antitumor enzyme L-lysine-α-oxidase has low immunogenicity. Antibodies not only reduce catalytic activity, but also affect the affinity of the enzyme with the substrate. Guinea pigs showed that native and modified L-lysine-α-oxidase at a dose of 35 U / kg in vivo and in vitro has a weak allergenic activity.