Properties of methicillin activity with sensitized methicillin resistant Staphylococcus aureus in presence of human alpha-lactalbumin made lethal to tumor cells in infected wound healing in diabetes: An animal model study

Abstract

Background: Wound healing and other tissue abnormalitiesare considered as major concerns in diabetes.The novel therapeutic options are urgently needed fortreatment of infections in diabetes caused by multidrugresistant S. aureus. Reportedly, HAMLET could sensitizebacterial pathogens to conventional antimicrobial agents.The objective of the present study was to evaluate woundhealing activity of methicillin in presence of HAMLETin methicillin resistant Staphylococcus aureus infectedwounds in diabetic rats.Materials & Methods: Fifty male diabetic rats were randomizedinto five groups of ten animals each. Inductionof diabetes was achieved using 60 mg/kg streptozotocin.In group I, 0.1 mL sterile saline 0.9% solution was addedto the wounds with no infection. In group II, the woundswere infected with MRSA and only treated with 0.1 mLthe sterile saline 0.9% solution. In group III, infectedwounds were treated with HAMLET (100 µg). In groupIV, animals with infected wounds were treated with 0.1mL topical application of 1 mg/mL methicillin. In groupV, animals with infected wounds were treated with topicalapplication of 0.1 mL solution of methicillin (1 mg/mL)and HAMLET (100 µg).Results: Microbiological examination, planimetric, histologicaland quantitative morphometric studies and determinationof hydroxyproline levels showed that there wassignificant difference between animals in group V comparedto other groups (P=0.001).Conclusions: HAMLET could make methicillin useful fortreatment of MRSA infected wounds in diabetes.