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Título : Actividad antiviral contra el virus de la fiebre amarilla, cepa vacunal 17D, de extractos de hojas de Taraxacum officinale GH Weber ex Wiggers
Autor : RODRÍGUEZ-ORTEGA, Morella
CHUMPITAZ, Zuriely
RÍOS, Santiago
MÉNDEZ, Mónica
MÉNDEZ, Jeannette
CABRERA, Gustavo
Palabras clave : Taraxacum officinale,
phytochemistry,
antiviral
Fecha de publicación : 6-Apr-2015
Resumen : Taraxacum officinale leaves were collected at two and 5 months of growth, for phytochemical studies and antiviral activity against flavivirus, using the 17D vaccine strain of yellow fever virus as a model. Using spectroscopy technique, a total of twelve (12) compounds were identified in the chloroform (C) and hexane (H) extracts of two and five months (2M and 5M) of recollection., The antiviral activity against the yellow fever 17D virus was evaluated with the plaque assay and the concentrations used (50 - 1,5 μg/mL) were no cytotoxic to Vero cells as determined using the MTT(3-(4,5-Dimetiltiazol-2yl)-2,4-difenilbromuro de tetrazolium) assay. The phytochemical composition of leaves growing for 5 months is different and more complex than leaves growing for 2 months. From the four extracts, only C5M inhibited the viral replication in a dose depend manner, with 100% viral inhibition at 50 μg/mL (p=0,0124) and the effective doses 50 (ED50: 10,2 ± 8,7 μg/mL), meanwhile, ED50 of C2M extract was 93,5 ± 23,5 μg/mL, thus, the extract C5M is 8 times more effective than extract C2M. The identified compounds in extract C5M were: Ψtaraxasteryl acetate, cafeic acid, taraxasteryl acetate, taraxerol, taraxerilo acetate and Ψ-taraxasterol. One of these compounds or the combinations of them is responsible for the reported high antiviral activity
URI : http://saber.ucv.ve/jspui/handle/123456789/8797
ISSN : 0717 7917
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