SABER UCV >
1) Investigación >
Artículos Publicados >

Por favor, use este identificador para citar o enlazar este ítem: http://hdl.handle.net/123456789/5111

Título : Anti-platelet effect of cumanastatin 1, a disintegrin isolated from venom of South American Crotalus rattlesnake
Autor : Da Silva, Manuel
Lucena, Sara
Aguilar, Irma
Rodríguez-Acosta, Alexis
Salazar, Ana M
Sánchez, Elda E
Girón, Maria E
Carvajal, Zoila
Arocha-Piñango, Carmen L
Guerrero, Belsy
Palabras clave : platelet adhesion
mass spectrometry
Disintegrin
Crotalus d.cumanensis
platelet aggregation
rattlesnakes
Fecha de publicación : 27-Nov-2013
Citación : Thrombosis Research;123:731–739 (2009).
Resumen : Disintegrins have been previously described in the venom of several snake families inhibiting signal transduction, cell-cell interactions, and cell-matrix interactions and may have therapeutic potential in heart attacks, thrombotic diseases, and cancers. This investigation describes the first disintegrin isolated from South American Crotalus venom (Venezuelan rattlesnake Crotalus durissus cumanensis), which inhibits platelet adhesion to matrix proteins. C. d. cumanensis crude venom was first separated on a Sephadex G-100 column into 4 fractions (SI to SIV). Crude venom and SIII fraction significantly diminished platelet adhesion to fibrinogen (Fg) and to fibronectin (Fn). Anti-adhesive SIII fraction was further separated by DEAE-Sephacel followed by C-18 reverse phase high performance liquid chromatography (HPLC). The platelet anti-adhesive fraction obtained was designated as cumanastatin-1. This disintegrin has a mass of 7.442 kDa as determined by mass spectrometry (MALDI-TOF/TOF) and pI of 8.5. Cumanastatin-1 also inhibited ADP-induced platelet aggregation with an IC50 of 158 nM. However, it did not significantly inhibit collagen and thrombin-induced platelet aggregation. Cumanastatin-1 considerably inhibited anti-αIIbβ3 integrin binding to platelets in a dose-dependent manner; however, it did not present any effect on the α5β1 integrin or on P-selectin.
URI : http://saber.ucv.ve/jspui/handle/123456789/5111
Aparece en las colecciones: Artículos Publicados

Ficheros en este ítem:

Fichero Descripción Tamaño Formato
Characterization of toxins from the broad-banded.pdf516.35 kBAdobe PDFVisualizar/Abrir

Los ítems de DSpace están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.

 

Valid XHTML 1.0! DSpace Software Copyright © 2002-2008 MIT and Hewlett-Packard - Comentarios