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Título : Anti-VSG antibodies induce an increase in Trypanosoma evansi intracellular Ca2+ concentration.
Autor : MENDOZA, M
Uzcanga, Graciela
PACHECO, R
ROJAS, H
CARRASQUEL, L
GARCIA-MARCHAN, Y
SERRANO-MARTI, X
Benaím, Gustavo
BUBIS, J
MIJARES, A
Palabras clave : antibodies
calcium
complement
Trypanosoma evansi
Trypanosoma vivax
variant surface glycoproteins
Fecha de publicación : 2008
Editorial : Parasitology
Citación : Vol. 135, Nº 11, pp.1303–1315.
Resumen : Trypanosoma evansi and Trypanosoma vivax have shown a very high immunological cross-reactivity. Anti-T. vivax antibodies were used to monitor changes in the T. evansi intracellular Ca2+ concentration ([Ca2+]i) by fluorometric ratio imaging from single parasites. A short-time exposure of T. evansi parasites to sera from T. vivax-infected bovines induced an increase in [Ca2+]i, which generated their complete lysis. The parasite [Ca2+]i boost was reduced but not eliminated in the absence of extracellular Ca2+ or following serum decomplementation. Decomplemented anti-T. evansi VSG antibodies also produced an increase in the parasite [Ca2+]i, in the presence of extracellular Ca2+. Furthermore, this Ca2+ signal was reduced following blockage with Ni2+ or in the absence of extracellular Ca2+, suggesting that this response was a combination of an influx of Ca2+ throughout membrane channels and a release of this ion from intracellular stores. The observed Ca2+ signal was specific since (i) it was completely eliminated following pre-incubation of the anti-VSG antibodies with the purified soluble VSG, and (ii) affinity-purified anti-VSG antibodies also generated an increase in [Ca2+]i by measurements on single cells or parasite populations. We also showed that an increase of the T. evansi [Ca2+]i by the calcium A-23187 ionophore led to VSG release from the parasite surface. In addition, in vivo immunofluorescence labelling revealed that anti-VSG antibodies induced the formation of raft patches of VSG on the parasite surface. This is the first study to identify a ligand that is coupled to calcium flux in salivarian trypanosomes.
URI : http://saber.ucv.ve/jspui/handle/123456789/4626
ISSN : 1.469-8161 (electrónica)
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