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Título : Ceramide increase cytoplasmic Ca2+ concentration in Jurkat T cells by liberation of calcium from intracellular stores and activation of a store-operated calcium channel
Autor : Colina, C
Flores, F
Rojas, H
Acosta, A
Castillo, C
Rosario, M
Israel, A
DiPolo, R
Benaím, Gustavo
Palabras clave : Ceramide
Calcium
Ceramine
Capacitative calcium entry channel
InsP3
Jurkat T cells
Fecha de publicación : Feb-2005
Editorial : Archives of Biochemistry and Biophysics
Citación : Vol. 436, pp. 333–345
Resumen : The effect of ceramide on the cytoplasmic Ca2+ concentration ([Ca2+]i) varies depending on the cell type. We have found that in Jurkat human T cells ceramide increases the [Ca2+]i from a thapsigargin-sensitive calcium pool and the subsequent activation of a capacitative Ca2+ entry. This effect occurs both in the presence and in the absence of extracellular calcium. Addition of ceramine, a non-hydrolysable analogue of ceramide, reproduced its effect on the [Ca2+]i ruling out that this is due to the conversion of ceramide to sphingosine. The effect of ceramide was additive to that obtained by sphingosine, but not to the Jurkat T cells specific antibody OKT3. However, different to the latter, ceramide do not induced an elevation of InsP3. The opening of a store operated Ca2+ channel by ceramide was corroborated by experiments of Fura-2 quenching, using Mn2+ as a surrogate for Ca2+ and confirmed by whole-cell recording patch clamp techniques.
URI : http://saber.ucv.ve/jspui/handle/123456789/4623
ISSN : 0003-9861
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