SABER UCV >
1) Investigación >
Artículos Publicados >

Por favor, use este identificador para citar o enlazar este ítem: http://hdl.handle.net/123456789/4200

Título : Leishmania braziliensis: Cytotoxic and chemotactic effects of branched chain polypeptide conjugates with poly [L-Lysine] backbone
Autor : Diaz, Emilia
Kohiday, Laszlo
Rios, Arturo
Vanegas, Oriana
Silva, Adrian
Szabo, R
Mezo, G
Hudecz, H
Ponte-Sucre, Alicia
Palabras clave : Leishmania
Chemotaxis
MTX-conjugates
Fecha de publicación : 19-Sep-2013
Citación : Experimental Parasitology;135:134-141, 2013
Resumen : Chemotactic responses play a significant role during Leishmania differentiation, as well as in the course of parasite–host–cell interaction, a process that precedes a successful infection. The present study uses the modified ‘‘two-chamber capillary assay’’ to quantitatively evaluate the chemotactic properties and the toxic activities of methotrexate containing branched chain polymeric polypeptide conjugates in Leishmania. Our results demonstrate that this methodology quantitatively determines the taxis of Leishmania towards/against gradients of compounds. They also demonstrate that chemotaxis produced by the polypeptide– methotrexate conjugates depends on specific chemical characteristics. For example, the N-terminal amino acid (Ser or Glu) location at the branch significantly influences the elicited chemotaxis. Furthermore, the use of different attachment sites in the methotrexate conjugates (a- or c-carboxylic groups) affect their chemotactic activity. Specific cytotoxic activities and cytostatic effects of the conjugates on parasites and on murine and human cells of the macrophage/monocyte system respectively, suggest that these ligands may be used as a group of anti-Leishmania substances acting selectively on Leishmania and different hosts.
URI : http://saber.ucv.ve/jspui/handle/123456789/4200
ISSN : 0014-4894
Aparece en las colecciones: Artículos Publicados

Ficheros en este ítem:

Fichero Descripción Tamaño Formato
Diaz et al 2013.pdf1.06 MBAdobe PDFVisualizar/Abrir

Los ítems de DSpace están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.

 

Valid XHTML 1.0! DSpace Software Copyright © 2002-2008 MIT and Hewlett-Packard - Comentarios