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Título : Plasmid-encoded resistance to arsenic compounds in Gram-negative bacteria isolated from a hospital environment in Venezuela1
Autor : Pedroza, Raquel
Ramirez, Alvaro
Alonso, Guillermina
Rodriguez, Vidal
Palabras clave : Plasmids
Amikac:in
Arsenite
R factors
Incompatibility
Rep probes
Fecha de publicación : 14-Jan-2013
Resumen : Resistance to arsenic compounds was examined among amikacin resistant Gram-negative bacteria isolate from a hospital environment. Arsenite resist,lnce (Ars’) was found in a high proportion of isolates ( > 60%) being frequently associated with resistance to tellurite (40%), and to other antimicrobial agents. Ars determinants (27%) were found to be transferable to E. coli K12 strains from which large plasmid DNA molecules were isolated and characterized by agarose gel electrophoresis. Plasmids were identified by both classical incompatibility tests, and by replicon typing using DNA specific probes. Most of the amikacin-arsenite (AkkArsIl conjugative plasmids belong to the H incompatibility group. These results suggest that Ak-Ars resistance linked to IncH plasmids is wide spread in Gram-negative bacteria. 0 1997 Elsevier Science B.V.
URI : http://saber.ucv.ve/jspui/handle/123456789/2363
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