SABER UCV >
1) Investigación >
Artículos Publicados >

Por favor, use este identificador para citar o enlazar este ítem: http://hdl.handle.net/123456789/1686

Título : New promising Euphorbiaceae extracts with activity in human lymphocytes from primary cell cultures
Autor : Llanes-Coronel, Doris S.
Gámez-Díaz, Laura Y.
Quintero-Suarez, Leidy P.
Páez, Lady J.
Torres, Fernando
Echeverri, Fernando
Ponte-Sucre, Alicia
Patiño, Pablo J.
Trujillo-Vargas, Claudia M.
Palabras clave : Natural Products;
Lymphoproliferation
Immunomodulation
Cytotoxicity
Apoptosis
Fecha de publicación : 2-Aug-2012
Citación : Immunopharmacology and Immunotoxicology;33:279-90, 2011
Resumen : CONTEXT: Euphorbiaceae plants exhibit anti-inflammatory and immunomodulatory properties. METHODS: We evaluated the activity of 14 extracts from seven Euphorbiaceae plants on primary immune cell cultures from healthy individuals. Peripheral blood mononuclear cells (PBMC) were exposed to the extracts w/o phytohaemagglutinin A or cycloheximide as agents that induce proliferation or apoptosis in PBMC, respectively. RESULTS: We found that five up to 14 Euphorbiaceae's extracts had the ability to modulate at least one of the immune parameters evaluated in this study. However, only the latex extracts of Euphorbia cotinifolia and Euphorbia tirucalli strongly induced both proliferation and apoptosis in PBMC. These extracts were further subfractioned by silica gel column chromatography. Two subfractions with enhanced activity in comparison to the crude extracts were obtained. Although these subfractions induced proliferation on both CD3(+) and CD3(-) cells, the most prominent effects were observed in the former subpopulation. Interestingly, the subfraction from E. tirucalli induced lymphocyte proliferation without the need of accessory cells; this ability was not inhibited by the carbohydrates d-galactose and α-Methyl-D-Mannopyranoside. CONCLUSIONS: Altogether, these results reveal the presence of novel candidates within the Euphorbia plants to induce proliferation and apoptosis in human lymphocytes, mainly in CD3(+) T cells.
URI : http://saber.ucv.ve/123456789/1686
ISSN : 0892-3973 (print)
1532-2513 (electronic)
Aparece en las colecciones: Artículos Publicados

Ficheros en este ítem:

Fichero Descripción Tamaño Formato
LLanes Coronel et al. 2011.pdf909.44 kBAdobe PDFVisualizar/Abrir

Los ítems de DSpace están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.

 

Valid XHTML 1.0! DSpace Software Copyright © 2002-2008 MIT and Hewlett-Packard - Comentarios