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|Título : ||PINDOLOL DECREASES PLASMA ANGIOTENSIN CONVERTING ENZYME ACTIVITY IN YOUNG SPONTANEOUSLY HYPERTENSIVE RATS|
|Autor : ||Niwa, MASAMI|
Stern de Israel, Anita
|Fecha de publicación : ||1984|
|Editorial : ||Clinic and exper. Theory and practice|
|Resumen : ||The renin-angiotensin systen is control led by a number of factors,
among which beta-adrenoceptors are wel I understood. In
turn, the antihypertensive action of bet a- ad renoc eptor blockers
may be partial'ly due to antagonism of the renin-angiotensin systern
(l ). Alterations of the renin-angiotensin system occur in spontaneously
hypertensive rats (SHR). These anirnals show low angjotensjn
converting enzj/me (ACE) activity in plasma (2). It has
been postulated that plasma ACE is orjginated in lung, although
endothelial cells in peripheral vessels could also make a contribution
(3), Studies have shown that angiotensin II localiy produced
'in arterial tissues by ACE might be important jn maintaining
vascul ar tone (4).
We stud'ied the effects of pindolol , a non-selective beta-adrenoceptor
blocker and 'lidely used anti hypertens ive drug, on plasma,
lung and mesenteric artery ACt of young (4 to s-week-old, pre or
early hypertensive) and adult (24 to 25-week-old) SHR.
Pindolol was given oral ly for 7 days once a day at a dose of l0
mg/kg body we'ight to male,4- and 24-week-old SHR and ldistar Kyoto
(I,JKY) rats, Animals were decapitated 24 hr after the last treatment.
ACE activity was measured by a radiochemical method (5).
Lung and mesenteric arteries were homogenized jn 0.1 14 potassium
phosphate buffer pH 8.0, by glass to g'lass tissue grinders. The
homogenate was centrifuged at 130,000 x g for 30 minutes, yie'ldinq
a pellet and a supernatant. The supernatant was considered
to óe a loosely-bound (soluble) ACE containing fraction (F1 ).
The pellet was resuspended in the buffer, containing 0.5% friton
X-100, ultrasonicated, and frozen-thawed 3 times in order to solubilize
the particulate enzyme (6). The homogenate was centrifuged
at 10,000 x g for 30 minutes; the supernatant was referred
as the F2 fraction (particulate).|
|URI : ||http://saber.ucv.ve/123456789/162|
|Aparece en las colecciones: ||Artículos Publicados|
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