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Título : Phosphatidylethanol stimulates the plasma-membrane calcium pump from human erythrocytes
Autor : SUJU, Meylin
DAVILA, Marbelly
POLEO, German
DOCAMPO, Roberto
BENAÍM, Gustavo
Palabras clave : Phosphatidylethanol
plasma-membrane calcium
human erythrocytes
ethanol catalysed
mammalian cells
natural lipidic
Fecha de publicación : 1996
Editorial : The Biochemical journal
Citación : Vol. 317;pp 933-938
Resumen : Phosphatidylethanol is formed by `transphosphatidylation' of phospholipids with ethanol catalysed by phospholipase D and can be accumulated in the plasma membrane of mammalian cells after treatment of animals with ethanol. In the present work we show that phosphatidylalcohols, such as phosphatidylethanol and phosphatidylbutanol, produced a twofold stimulation of the Ca2+-ATPase activity of human erythrocytes. This stimulation occurs with the purified, solubilized enzyme as well as with ghost preparations, where the enzyme is in its natural lipidic environment and is different to that obtained with other acidic phospholipids such as phosphatidylserine. Addition of either phosphatidylserine, phosphatidylethanol or phosphatidylbutanol to the purified Ca2+-ATPase, or to ghosts preparations, increased the affinity of the enzyme for Ca2+ and the maximal velocity of the reaction as compared with controls in the absence of acidic phospholipids. However, in contrast with what occurs with phosphatidylserine, simultaneous addition of phosphatidylalcohols and calmodulin increased the affinity of the enzyme for Ca2+ to a greater extent than each added separately. When ethanol was added to either the purified erythrocyte Ca2+-ATPase or to erythrocyte-ghost preparations in the presence of acidic phospholipids, an additive effect was observed. There was an increase in the affinity for Ca2+ and in the maximal velocity of the reaction, well above the values obtained with ethanol or with the acidic phospholipids tested separately. These findings could have pharmacological importance. It is conceivable that the decrease in the intracellular Ca2+ concentration that has been reported in erythrocytes as a result of ethanol intoxication could be due to the stimulation of the Ca2+-ATPase by the accumulated phosphatidylethanol, to a direct effect of ethanol on the enzyme or to an additive combination of both.
Descripción : Gustavo Benaim. Instituto de Biologia Experimental, Facultad de Ciencias, Universidad Central de Venezuela, Apartado 47114, Caracas, Venezuela, and Department of Veterinary Pathobiology, University of Illinois at Urbana-Champaign, IL 61801, U.S.A. Correo electrónico: gbenaim@idea.gob.ve
URI : http://hdl.handle.net/123456789/15706
ISSN : 1470-8728 (Electronic)
0264-6021 (Linking)
0264-6021 (Print)
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