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Título : Disruption of Ca2 + Homeostasis in Trypanosoma cruzi by Crystal Violet
Autor : DOCAMPO, ROBERTO
GADELHA, FERNANDA R.
MORENO, SILVIA N. J.
BENAÍM, GUSTAVO
HOFFMANN, MARIA E.
VERCESI, ANIBAL E.
Palabras clave : Mitochondria
plasma membrane Ca2+-ATPase
fura-2
crystal violet
mitochondrial membrane
Trypanosoma cruzi epimastigotes
Fecha de publicación : 1993
Editorial : The Journal of eukaryotic microbiology
Citación : Vol. 40;No. 3 pp 311-316
Resumen : We have demonstrated previously that crystal violet induces a rapid, dose-related collapse of the inner mitochondrial membrane potential of Trypanosoma cruzi epimastigotes. In this work, we show that crystal violet-induced dissipation of the membrane potential was accompanied by an efflux of Ca2+ from the mitochondria. In addition, crystal violet inhibited the ATP-dependent, oligomycin-, and antimycin A-insensitiveCa2+ uptake by digitonin-permeabilized epimastigotes. Crystal violet also induced Ca2+ release from the mitochondria and endoplasmic reticulum of digitonin-permeabilized trypomastigotes. Furthermore, crystal violet inhibited Ca2+ uptake and the (Ca2+ -Mg2+)-ATPase of a highly enriched plasma membrane fraction of epimastigotes, thus indicating an inhibition of other calcium transport mechanisms of the cells. Disruption of Ca2+ homeostasis by crystal violet may be a key process leading to trypanosome cell injury by this drug.
URI : http://hdl.handle.net/123456789/15658
ISSN : 1550-7408 (Electronic)
1066-5234 (Linking)
1066-5234 (Print)
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