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Título : Dissociation of Purified Erythrocyte Ca2+-ATPaseb y Hydrostatic Pressure
Autor : Coelho-Sampaio, Tatiana
Ferreira, Sergio T.
Benaím, Gustavo
Vieyra, Adalberto
Palabras clave : erythrocyte plasma membranes
fluorescence spectroscopy
highpressure techniques
intrinsic fluorescence emission
fluorescence polarization
atmospheric pressure
Fecha de publicación : 1991
Editorial : THE JOURNAL OF BIOLOGICAL CHEMISTRY
Citación : Vol. 266;No. 3 pp 22266-22272
Resumen : Subunit interactions in the Ca2+-ATPase from erythrocyte plasma membranes were investigated through a combination of fluorescence spectroscopy and highpressure techniques. Application of hydrostatic pressure in the range of 1 bar to 2.4 kbar promoted full dissociation of the ATPase, as revealed by spectral shifts of the intrinsic fluorescence emission and by changes in the fluorescence polarization of dansyl-conjugated ATPase. Pressure dissociation of the ATPase displayed a dependence on protein concentration compatible with dissociation of a dimer. Calculated from pressure-dissociation curves, the standard volume change dVo for the association of subunits was 43-50 ml/mol and KO, the dissociation constant at atmospheric pressure, was 6-9 X l0-8 M. Addition of Ca2+ tabilized the dimeric ATPase structure against pressure dissociation, whereas addition of vanadate facilitated dissociation by pressure. These results suggest that intersubunit interactions depend on the equilibrium between the two major conformational states E1 and E2 of the ATPase. Addition of calmodulin in the presence of Ca2+ had no additional effect when compared to that observed in the presence of Ca2+a lone. This finding is interpreted in terms of the mechanism of Calmodulin activation of ATPase catalysis.
URI : http://hdl.handle.net/123456789/15645
ISSN : 1083-351X
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