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Título : The Effect of Ethanol on the Plasma Membrane Calcium Pump Is Isoform-specific
Autor : Cervino, Vincenza
Benaím, Gustavo
Carafoli, Ernesto
Guerini, Danilo
Palabras clave : effect of ethanol
isoforms
plasma membrane
Ca2+
ATPase
baculovirus system
ethanol
PMCA4 protein
Fecha de publicación : 1998
Editorial : THE JOURNAL OF BIOLOGICAL CHEMISTRY
Citación : Vol. 273;No. 45 pp. 29811–29815
Resumen : The effect of ethanol has been studied on four different isoforms of the plasma membrane Ca2+-ATPase expressed in Sf9 cells with the help of the baculovirus system. The PMCA2CI protein was maximally activated by 0.5% ethanol, a concentration 8–10 times lower than that needed to obtain the same effect on the PMCA4 protein or on the pump of erythrocyte membranes, which is a mixture of isoforms 1 and 4. Experiments performed with truncated pumps indicated that the stimulation by ethanol was lost if the C-terminal region between Lys1065 and Lys1161, encompassing the Calmodulin binding domain, was removed. These observations indicate that the stimulation is the result of a direct interaction of ethanol with the C-terminal regulatory domain of the Ca2+ pump.
URI : http://hdl.handle.net/123456789/15643
ISSN : 1083-351X
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