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Título : Nitrotyrosine production following the interaction between autologous human B lymphocytes and natural killer cells
Autor : Márquez, María E.
Blanca, Isaac
Bianco Colmenares, Nicolás E.
De Sanctis, Juan B.
Palabras clave : B lyrnphocytes
natural killer cells
nitricoxide
nitrotyrosine
peroxynitrate
alIogeneic cells
Fecha de publicación : 1999
Editorial : Medical science research
Citación : Vol. 27;pp. 589- 590
Resumen : It has been suggested that nitric oxide (NO) and superoxide anion may be involved in heterotypic interactions. We have now assessed nitrotyrosine. one of the products of peroxynitrite (ONOO-), by a specific sandwich ELISA, in the supernatants of autologous B lymphocytes and natural killer (NK) cells that were cultured for 48h alone or mixed in different ratios (5: 1, 2 .5: 1 and 1: 1). There were no significant differences in nitrotyrosine concentration independently of the conditions used. However, when cultures were established with alIogeneic cells at a B: NK ratio of 1: 1, nitrotyrosine levels markedly increased from 11.2 ± 3.5 up to 32.0 ± 4.8 ng/ml (P < 0.000 1). We conclude that without alIogeneic stimuli, there is no net increase in nitrotyrosine production, suggesting that cell survival may depend on peroxynitrate production.
URI : http://hdl.handle.net/123456789/14963
ISSN : 0269-8951
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