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Título : Lipoproteins induce tyrosine phosphorylation in a murine macrophage cell line
Autor : Rivera, Henry
Radzioch, Danuta
Blanca, Isaac
Bianco Colmenares, Nicolás E.
De Sanctis, Juan B.
Palabras clave : Lipoproteins
chylomicrons
tyrosine phoshorylation
macrophage cell line
monoclonal antibody
phosphotyrosine
Fecha de publicación : 1998
Editorial : Medical science research
Citación : Vol. 26;pp. 535-537
Resumen : Lipoproteins chylomicrons (CM) very low density lipoprotein (VLDL). Low density lipoprotein (LDL) high density lipoprotein (HDL) and acetyl modified LDL (AcLDL) are able to stimulate macrophages upon binding lo specific receptors. Using flow cytometry and a specific monoclonal antibody against phosphotyrosine. We assessed the effect of lipoproteins on total intracellular expression of immunoreactive phosphotyrosine. Tyrosine phosphorylation increased when lipoproteins were added to a macrophage cell line which was previously cultured in RPM-0.50% fatty acid free bovine serum albumin. The optimal effect occurred at 15 min for all the lipoproteins used. The concentrations of lipoprotein were 10 μg of protein/ml of CM and 20 μg of protein/ml for the other lipoproteins. The effect of lipoproteins on phosphotyrosine positiveness was as follows: AcLDL> HDL > CM > VLDL > DL. Thus lipoproteins may activate macrophages through tyrosine phosphorylation and this signal transduction pathway seems to be enhanced when these cells are activated with AclDL.
URI : http://hdl.handle.net/123456789/14882
ISSN : 0269-8951
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