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Por favor, use este identificador para citar o enlazar este ítem: http://hdl.handle.net/123456789/14717

Título : High-density Ficoll-Hypaque as an alternative method of eosinophil purification
Autor : Conesa, Angela
Pérez, Carlos
Rivera, Henry
Aldrey, Oscar
Bianco Colmenares, Nicolás E.
De Sanctis, Juan B.
Palabras clave : normodense eosinophil
high-density Ficoll-Hypaque
eosinophil purification
mononuclear
polymorphonuclear cells
anti-CD16
hypodense eosinophils
leucocyte populations
Fecha de publicación : 1997
Editorial : Medical science research
Citación : Vol. 25;No. 11 pp 751-755
Resumen : A new method for normodense eosinophil purification is proposed. It is based on the use of high-density Ficoll-Hypaque (density = 1.114 g mL-1) instead of dextran sedimentation plus standard Ficoll-Hypaque, before discontinuous Percoll gradient centrifugation. Eosinophils from 11 controls and 11 eosinophilic patients were studied. The density distribution of eosinophils was similar in both groups. However, eosinophilic patients showed a significant increase in the absolute count of hypodense eosinophils. Normodense eosinophil purity and recovery were significantly higher in controls as compared with eosinophilic patients (p < 0.05). High-density Ficoll-Hypaque allowed separation of mononuclear and polymorphonuclear cells with high purity and recovery using a single centrifugation step. It also reduces one step in eosinophil purification as compared to the standard method. Thirdly, contrary to the single anti-CD16 magnetic bead separation method, it allows the isolation of normodense and hypodense eosinophils. In summary, this method is economic, reliable and permits the purification of different leucocyte populations.
URI : http://hdl.handle.net/123456789/14717
ISSN : 0269-8951
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