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Título : LED Xuorescence spectroscopy for direct determination of monoamine oxidase B inactivation
Autor : Castillo, Jimmy
Hung, Jeanette
Rodríguez, María del C.
Bastidas, Elsy
Laboren, Ivanna
Jaimes, Alba
Palabras clave : MAO-B
LED FLUORESCENCE
BERBERINE
Fecha de publicación : 16-Jun-2005
Editorial : Analytical Biochemistry
Citación : Analytical Biochemistry 343 (2005) 293–298
Resumen : In this work, we report an alternative assay for the determination of the inhibitory eVect on monoamine oxidase B (MAO-B) activity of probe compounds. Enzyme MAO-B exhibits Xuorescence emissions when it is excited at 412 nm. Using an inexpensive blue LED-like excitation source, we measured the quenching of Xuorescence intensity of MAO-B enzyme during the reaction with inhibitors. The applicability of the procedure is demonstrated by assays with L-deprenyl and berberine as inhibitors through the use of Xuorescence studies. The IC50 values of L-deprenyl and berberine were 0.04 and 90 M, respectively. The KI values were 0.020 and 47 M for L-deprenyl and berberine, respectively. These IC50 and KI values were similar to the values obtained with a standard method. These results demonstrate the feasibility of this method as an alternative to follow the inhibitory eVect on MAO-B.
URI : http://hdl.handle.net/123456789/14054
ISSN : 0003-2697
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