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Título : Immunocytochemical detection of Entamoeba histolytica
Autor : Pérez de Súarez, Eva
Pérez-Schael, Irene
Perozo-Ruggeri, Gladys
de Dávila, Delia
Römer, Hans
Tapia, Félix J.
Palabras clave : immunoglobulin
immunoperoxidase
protozoan
Trophozoites
microscope
cysts
Fecha de publicación : 1987
Editorial : Transactions of the Royal Society of Tropical Medicine and Hygiene
Citación : Vol. 81;Nº 4
Resumen : Human anti-Encamoeba histolytica immunoglobulin was used to detect Entamoeba histolytica in 74 positive samples from several different sources, using an indirect immunoperoxidase method. In 73 samples, the protozoan was easily identified. Trophozoites and cysts of all cultured Entamoeba strains examined were strongly stained and as few as 3 trophozoites per microscope slide could be detected. In addition, 51 negative control samples were also tested and non-specific reactions were not observed. These pre1iminary results show that this method is both sensitive and specific, and can easily detect trophozoites and cysts of different E. histolytica strains.
URI : http://saber.ucv.ve/jspui/handle/123456789/13285
ISSN : 1878-3503
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