SABER UCV >
1) Investigación >
Artículos Publicados >

Por favor, use este identificador para citar o enlazar este ítem: http://hdl.handle.net/123456789/13239

Título : Muscarinic agents modify kinetics properties of membrane-bound guanylyl cyclase activity I.
Autor : Alfonzo R., Marcelo J.
Gonzalez de Alfonzo, Ramona
Lippo de Becemberg, Itala
Pefia de Aguilar, A.E.
Camarillo, I
Palabras clave : membrane-bound
guanylyl cyclase
Muscarinic agents
NaCI inhibition
Muscarinic receptor
Enzyme-receptor complex
Plasma membrane
Tracheal smooth muscle
Fecha de publicación : Aug-1989
Editorial : FEBS LETTERS
Citación : Vol. 253;1,2
Resumen : Plasma membranes from bovine tracheal smooth muscle show guanylyl cyclase activity, which can be stimulated by muscarinic agonists such carbamylcholine and oxotremorine and blocked by atropine. This stimulation was observed in the presence of 150 mM NaCI. In the absence of this salt, guanylyl cyclase activity was considerably higher but was not affected by muscarinic agonists. Carbamylcholine decreased the apparent K,~ but did not change the Vm~ of this enzyme. When plasma membrane fractions were extracted with 1% octylglucoside, guanylyl cyclase activity was preserved, however the muscarinic activation was abolished, despite a muscarinic receptor capable of [aH]quinuclidinylbenzilate binding being present in the extract. The detergent extraction changed the affinity of guanylyl cyclase for GTP but the Mn z+ kinetics was unaltered. Based on these findings and on current information in the literature, we propose that another component is required to restore the link between the muscarinic receptor and guanylyl cyclase, however the nature of this component remains to be established.
URI : http://saber.ucv.ve/jspui/handle/123456789/13239
ISSN : 0014-5793
Aparece en las colecciones: Artículos Publicados

Ficheros en este ítem:

Fichero Descripción Tamaño Formato
Referencia # 11 FEBS 89.pdf478.02 kBAdobe PDFVisualizar/Abrir

Los ítems de DSpace están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.

 

Valid XHTML 1.0! DSpace Software Copyright © 2002-2008 MIT and Hewlett-Packard - Comentarios