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Título : Immunologic evaluation and validation of metods using synthetic peptides derived from Mycobacterium tuberculosis for the diagnosis of tuberculosis infection
Autor : Araujo, Zaida
Giampietro, Francesca
Bochichio, Marias de los Angeles
Palacios, Andrea
Dinis, Jenifer
Isern, Jaime
De Waard, Jacobus H.
Rada, Elsa
Borges, Rafael
Fernandez de Larrea, Carlos
Villasmil, Angel
Vanegas, Magnolia
Enciso-Moreno, Jose Antonio
Patarroyo, Manuel Alfonso
Palabras clave : pulmonary tuberculosis
extrapulmonary tuberculosis
synthetic peptide
ESAT-6 antigen
Ag85A antigen
Fecha de publicación : 7-Nov-2012
Editorial : Mem Inst Oswaldo Cruz 2013;108(2):131-139
Resumen : The goal of this study was to demonstrate the usefulness of an enzyme-linked immunosorbent assay (ELISA) for the serodiagnosis of pulmonary tuberculosis (PTB) and extrapulmonary TB (EPTB). This assay used 20 amino acid-long, non-overlapped synthetic peptides that spanned the complete Mycobacterium tuberculosis ESAT-6 and Ag85A sequences. The validation cohort consisted of 1,102 individuals who were grouped into the following five diagnostic groups: 455 patients with PTB, 60 patients with EPTB, 40 individuals with non-EPTB, 33 individuals with leprosy and 514 healthy controls. For the PTB group, two ESAT-6 peptides (12033 and 12034) had the highest sensitivity levels of 96.9% and 96.2%, respectively, and an Ag85A-peptide (29878) was the most specific (97.4%) in the PTB groups. For the EPTB group, two Ag85A peptides (11005 and 11006) were observed to have a sensitivity of 98.3% and an Ag85A-peptide (29878) was also the most specific (96.4%). When combinations of peptides were used, such as 12033 and 12034 or 11005 and 11006, 99.5% and 100% sensitivities in the PTB and EPTB groups were observed, respectively. In conclusion, for a cohort that consists entirely of individuals from Venezuela, a multi-antigen immunoassay using highly sensitive ESAT-6 and Ag85A peptides alone and in combination could be used to more rapidly diagnose PTB and EPTB infection.
URI : http://saber.ucv.ve/jspui/handle/123456789/13010
ISSN : 1678-8060
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