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Título : Isolation and molecular identification of Leishmania chagasi from a bat (Carollia perspicillata) in northeastern Venezuela
Autor : De Lima, Hector
Rodríguez, Noris
Barrios, Miguel. A
Ávila, Ángela
Cañizales, Israel
Gutiérrez, Saúl
Palabras clave : Leishmania chagasi
bats
molecular biology
reservoir
Venezuela
Fecha de publicación : Jun-2008
Editorial : Mem Inst Oswaldo Cruz, Rio de Janeiro
Citación : Vol.103;4
Resumen : This report describes the isolation of a Leishmania chagasi strain from a bat (Carollia perspicillata), and its identification using biological methods and molecular characterization. The parasites were isolated in an artificial culture medium from a blood sample extracted from a bat heart. The isolate was then inoculated into the footpads of Balb/c mice, which subsequently developed a typical nodular leishmanial lesion; the parasites were confirmed as Leishmania by smear and histopathology. Molecular characterization of the parasites was performed by polymerase chain reaction with species-specific primers, kDNA restriction pattern following Hae III endonuclease digestion and dot blot hybridization using a kDNA probe. This report demonstrates that bats can be hosts for L. chagasi species and suggests the need for studies to determine whether they may be involved in foci of visceral leishmaniasis.
URI : http://saber.ucv.ve/jspui/handle/123456789/12688
ISSN : 1678-8060
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