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Título : Determination of the cytokine profile in American cutaneous leishmaniasis using the polymerase chain reaction
Autor : CACERES-DITTMAR, G.
Tapia, Félix J.
SANCHEZ, M. A.
YAMAMURA, M.
UYEMURA, K.
MODLIN, R. L.
BLOOM, B. R.
CONVIT, J.
Palabras clave : cytokine
leishmaniasis
polymerase chain reaction
T lymphocytes
Fecha de publicación : Apr-1993
Editorial : Clin Exp Immunol
Citación : Vol. 91;
Resumen : The lymphokine profiles were determined in the skin lesions of the three distinct clinical forms of American cutaneous leishmaniasis (ACL), using a reverse transcriptase polymerase chain reaction (RT-PCR) and primers for various lymphokines. The message for interferon-gamma (IFN-y), tumour necrosis factor-beta (TNF-fl), and IL-8 was expressed in the three clinical forms of ACL. IL-lI# mRNA was expressed in most localized (LCL) and mucocutaneous (MCL) leishmaniasis, but in only few of the diffuse cutaneous leishmaniasis (DCL). IL-2 mRNA was detected in about half of the lesions, with more prominent values for MCL. IL-4 mRNA was present in most lesions from the three clinical forms, but markedly increased in DCL. IL-5 and IL-10 mRNAs were expressed in all MCL and in half of the DCL lesions and weakly expressed in LCL lesions. IL-10 mRNA was more abundant in MCL lesions. In contrast, IL-6 and TNF-ox mRNAs were expressed in a large number of LCL. In MCL, IL-6 mRNA was expressed in most cases and TNF-a mRNA in all the cases. In DCL, IL-6 mRNA was absent and TNF-c mRNA was weakly expressed. These results suggest that most T cells present in the MCL and DCL lesions secrete a mixture of type I and type 2 cytokine patterns, but in DCL granulomas type 2 cytokines predominate. In LCL the cytokine patterns show a mixture of type 1 and type 0 with a preponderance of IFN-y over IL-4, and low levels of IL-5 and IL-10. The lack of IL-6 and TNF-cx mRNAs, and the low expression of IL- 1I# in DCL lesions suggest a defect in the antigen-processing cells that may account for the state of unresponsiveness in these patients.
URI : http://saber.ucv.ve/jspui/handle/123456789/12495
ISSN : 1365-2249
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