SABER UCV >
1) Investigación >
Artículos Publicados >
Por favor, use este identificador para citar o enlazar este ítem:
|Título : ||Determination of the cytokine profile in American cutaneous leishmaniasis using the polymerase chain reaction|
|Autor : ||CACERES-DITTMAR, G.|
Tapia, Félix J.
SANCHEZ, M. A.
MODLIN, R. L.
BLOOM, B. R.
|Palabras clave : ||cytokine|
polymerase chain reaction
|Fecha de publicación : ||Apr-1993|
|Editorial : ||Clin Exp Immunol|
|Citación : ||Vol. 91;|
|Resumen : ||The lymphokine profiles were determined in the skin lesions of the three distinct clinical forms of
American cutaneous leishmaniasis (ACL), using a reverse transcriptase polymerase chain reaction
(RT-PCR) and primers for various lymphokines. The message for interferon-gamma (IFN-y),
tumour necrosis factor-beta (TNF-fl), and IL-8 was expressed in the three clinical forms of ACL.
IL-lI# mRNA was expressed in most localized (LCL) and mucocutaneous (MCL) leishmaniasis, but
in only few of the diffuse cutaneous leishmaniasis (DCL). IL-2 mRNA was detected in about half of
the lesions, with more prominent values for MCL. IL-4 mRNA was present in most lesions from the
three clinical forms, but markedly increased in DCL. IL-5 and IL-10 mRNAs were expressed in all
MCL and in half of the DCL lesions and weakly expressed in LCL lesions. IL-10 mRNA was more
abundant in MCL lesions. In contrast, IL-6 and TNF-ox mRNAs were expressed in a large number of
LCL. In MCL, IL-6 mRNA was expressed in most cases and TNF-a mRNA in all the cases. In DCL,
IL-6 mRNA was absent and TNF-c mRNA was weakly expressed. These results suggest that most
T cells present in the MCL and DCL lesions secrete a mixture of type I and type 2 cytokine patterns,
but in DCL granulomas type 2 cytokines predominate. In LCL the cytokine patterns show a mixture
of type 1 and type 0 with a preponderance of IFN-y over IL-4, and low levels of IL-5 and IL-10. The
lack of IL-6 and TNF-cx mRNAs, and the low expression of IL- 1I# in DCL lesions suggest a defect in
the antigen-processing cells that may account for the state of unresponsiveness in these patients.|
|URI : ||http://saber.ucv.ve/jspui/handle/123456789/12495|
|ISSN : ||1365-2249|
|Aparece en las colecciones: ||Artículos Publicados|
Los ítems de DSpace están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.