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Título : Colombistatin: a disintegrin isolat ed from the venom of the South American snake ( Bothrops colombiensis ) that e V ectively inhibits platelet aggregation and SK-Mel-28 cell adhesion
Autor : Sánchez, Elda E
Rodríguez-Acosta, Alexis
Palomar, Rene
Lucena, Sara E.
Bashir, Sajid
Soto, Julio G
Pérez, John C.
Palabras clave : Disintegrin
Bothrops colombiensis
T24 cells
Platelet aggregation
Cell migration
Fecha de publicación : 27-Nov-2013
Citación : Archives of Toxicology;83(3):271-279 (2009)
Resumen : Snake venoms are complex mixtures of proteins, which aVect the vital biologic systems of prey, as well as humans. Envenomation leads to immobilization by paralysis, cardiac, and circulatory failure. These same venom proteins that cause havoc in the physiologic system could be used as therapeutic agents. Disintegrins and disintegrin- like proteins are molecules found in the venom of four snake families (Atractaspididae, Elapidae, Viperidae, and Colubridae). The disintegrins are non-enzymatic proteins that inhibit cell–cell interactions, cell–matrix interactions, and signal transduction. These proteins may have potential in the treatment of strokes, heart attacks, cancers, osteoporosis, and diabetes. The present study describes the isolation and characterization of a disintegrin (colombistatin) found in the venom of the Venezuelan snake mapanare (Bothrops colombiensis). Colombistatin was puriWed by a two-step high-performance liquid chromatography procedure, which included reverse phase C18 and size exclusion protein Pak 60. Colombistatin inhibited ADP-induced platelet aggregation, human urinary (T24) and skin melanoma (SK-Mel-28) cancer cell adhesion to Wbronectin, and cell migration. Colombistatin contained 72 amino acids with a mass of 7.778 kDa as determined by mass spectrometry. Colombistatin could be used as a therapeutic tool in the treatment of melanoma cancers and also thrombotic diseases.
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