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Título : | Ceramide increase cytoplasmic Ca2+ concentration in Jurkat T cells by liberation of calcium from intracellular stores and activation of a store-operated calcium channel |
Autor : | Colina, C Flores, F Rojas, H Acosta, A Castillo, C Rosario, M Israel, A DiPolo, R Benaím, Gustavo |
Palabras clave : | Ceramide Calcium Ceramine Capacitative calcium entry channel InsP3 Jurkat T cells |
Fecha de publicación : | Feb-2005 |
Editorial : | Archives of Biochemistry and Biophysics |
Citación : | Vol. 436, pp. 333–345 |
Resumen : | The effect of ceramide on the cytoplasmic Ca2+ concentration ([Ca2+]i) varies depending on the cell type. We have found that in
Jurkat human T cells ceramide increases the [Ca2+]i from a thapsigargin-sensitive calcium pool and the subsequent activation of a
capacitative Ca2+ entry. This effect occurs both in the presence and in the absence of extracellular calcium. Addition of ceramine, a
non-hydrolysable analogue of ceramide, reproduced its effect on the [Ca2+]i ruling out that this is due to the conversion of ceramide
to sphingosine. The effect of ceramide was additive to that obtained by sphingosine, but not to the Jurkat T cells specific antibody
OKT3. However, different to the latter, ceramide do not induced an elevation of InsP3. The opening of a store operated Ca2+ channel
by ceramide was corroborated by experiments of Fura-2 quenching, using Mn2+ as a surrogate for Ca2+ and confirmed by
whole-cell recording patch clamp techniques. |
URI : | http://hdl.handle.net/10872/4623 |
ISSN : | 0003-9861 |
Aparece en las colecciones: | Artículos Publicados
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