Identification of a sphingosine-sensitive Ca2+ channel in the plasma membrane of Leishmania mexicana

Abstract

The disruption of the intracellular Ca2+ homeostasis of Leishmania mexicana represents a major target for the action of drugs, such as amiodarone and miltefosine. However, little is known about the mechanism of Ca2+ entry to these cells. Here we show the presence of a Ca2+ channel in the plasma membrane of these parasites. This channel has many characteristics similar to the human L-type voltage-gated Ca2+ channel. Thus, Ca2+ entry is blocked by verapamil, nifedipine and diltiazem while Bay K 8644 opened this channel. However, different to its human counterpart, sphingosine was able to open this channel, while other well known sphingolipids had no effect. This fact could have important pharmacological implications.