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|Título : ||Improvement in the Diagnosis of Tuberculosis Combining Mycobacterium Tuberculosis Immunodominant Peptides and Serum Host Biomarkers|
|Autor : ||Lopez-Ramos, Juan E.|
Castañeda-Delgado, Julio E.
Lara-Ramirez, Edgar E.
Serrano, Carmen J.
Enciso-Moreno, Jose A.
|Palabras clave : ||Tuberculosis|
Host response biomarkers
|Fecha de publicación : ||9-Jul-2018|
|Editorial : ||Archives of Medical Research|
|Citación : ||Araujo. Z Et All Improvement in the Diagnosis of Tuberculosis Combining Mycobacterium Tuberculosis Immunodominant Peptides and Serum Host Biomarkers Archives of Medical Research Volume 49, numero3|
|Citación : ||;3|
|Resumen : ||Background. Pulmonary tuberculosis (PTB) is a public health problem with 10.4 million
new cases reported in 2017 (1). According to the World Health Organization (WHO), ac-
curate diagnostic tests based in serum biomarkers to detect new cases of tuberculosis are
Aim of the study. To evaluate antibodies against Mycobacterium. tuberculosis (Mtb) pep-
tides (Ab-Mtb) and three soluble host biomarkers by ELISA serial multiple test in sera
from non-infected controls (NIC, n 5 31), latent tuberculosis (LTB, n 5 37) and PTB
(n 5 28) patients in a diagnosis tuberculosis assay. Materials and methods. Levels of four Ab-Mtb peptides derived from Mtb and three host response molecules in serum from NIC, LTB and PTB were evaluated by ELISA as tuber-
culosis biomarkers. Multiple comparisons tests, determination of diagnostic values and
ROC curves were performed. Serial and parallel multiple tests were performed with
the biomarkers with the highest discriminatory capacity to improve diagnostic values of the test. Results. We found significant differences between biomarkers levels in PTB comparing LTB and NIC to all candidate biomarkers; peptides P12033, P12037, and serum bio- markers such as sCD14 and chemokine CXCL9 showed the best sensitivity and speci-ficity, the highest discriminatory power, and the best area under the curve (AUC) individually. In serial multiple tests, P12037 and sCD14 together have 92% of sensitivity and 91% of specificity, with positive and negative likelihood ratios greater than 10. Conclusions. Ab-Mtb peptide P12037 and sCD14 could be applied in a diagnostic test
for suspected PTB to improve accuracy and time to diagnosis and could be implemented
in a POCT device which can be affordable. 2018 IMSS. Published by Elsevier Inc.|
|URI : ||http://hdl.handle.net/10872/19546|
|ISSN : ||0188-4409|
|Aparece en las colecciones: ||Artículos Publicados|
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